Part:BBa_K1396000:Design

pSB1C3: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 985
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 232
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1634
  

Design Notes

We capped the LL-37 Domain which is otherwise toxic to cells with a removable strep tag that can be used for column chromatography. Additionally there are linker units between individual components

Source

Synthesized in lab